School of Life & Health Sciences Aston University Aston TriangleBirmingham B4 7ETUK
Room: MB 438Q Tel: +44 (0) 121 204 3264 Email: firstname.lastname@example.org
Dr Johnson received her PhD in 2007 from McMaster University in Hamilton, Ontario, Canada with a focus on the biology of allergic asthma. She subsequently moved to the Karolinska Institute in Stockholm, Sweden as a postdoctoral fellow in the laboratory of Dr Jonas Fuxe to perform studies on the transcriptional regulation of epithelial-mesenchymal transition in cancer and allergic asthma. Dr Johnson then took up a second postdoctoral fellowship carried out in the laboratory of Prof Qutayba Hamid at the Meakins-Christie Laboratories at McGill University in Montréal, Québec, Canada to investigate epithelial-mesenchymal transition in primary bronchial epithelial cells derived from healthy subjects and severe asthmatics. Dr Johnson then held a Research Fellow position at Imperial College London from December 2011 to April 2016 in the Leukocyte Biology section of the National Heart and Lung Institute, Faculty of Medicine where she began studying the role of tissue-resident mesenchymal progenitor cells (pericytes) in driving lung fibrosis.
Other activities include working as a freelance proofreader, teaching immunology at the undergraduate and graduate levels, and performing peer reviews for a number of journals in the respiratory field and in general biological sciences. Jill has organized and taken part in an number of public engagement events such as Science Uncovered at the Natural History Museum, MRC Centenary events at the Science Museum and Southbank Observation Point, and Synapse with Girlguiding, and is a member of ScienceGrrl, a grassroots organisation celebrating and supporting women in science.
PhD Supervisor: Manel Jordana, MD PhDResearch Project: Immunological and pharmacological intervention in a novel mouse model of chronic allergic airway disease
BY1IM1 – Introductory ImmunologyBY2PA2 – Molecular PathologyBY3BD2 – Biological Basis of Human DiseaseBI4005 – Human DiseaseBI4103 – Transplantation BiologyBI4106 – Tissue regeneration
Dr Johnson’s research focuses on the role of vascular pericytes in allergic asthma, specifically the progenitor cell capacity of these cells and their contribution to asthma pathogenesis. One of the hallmarks of asthma, airway remodelling, is thought to be facilitated by a chronic inflammatory process driven by allergen exposure. Airway remodelling results in irreversible modifications to structural cells of the airway, ultimately leading to airway narrowing and breathing difficulties. However, unanswered questions remain regarding the cell types that contribute to remodelling, the influence of growth factors and the recruitment of lung-resident progenitor cells.
These areas of interest led Jill to investigate pericytes and their roles in asthma. Pericytes are elongated, branched mesenchymal cells that support blood vessels. Pericytes have been described as tissue-specific mesenchymal progenitor cells, and represent an opportunity to extend our knowledge of adult stem cells to understand disease pathogenesis and potential therapeutic interventions.
In the Johnson lab, an established mouse model of asthma driven by chronic respiratory exposure to house dust mite (HDM) is used to induce chronic allergic asthma in a way that closely mimics human asthma. We have demonstrated that, following chronic HDM exposure, pericytes detach from the microvasculature and accumulate within the smooth muscle bundles of the airways, thus contributing to airway remodelling and lung dysfunction (Johnson et al., AJP Lung 2015). Pericytes in the respiratory microvasculature are evaluated in control mice as well as in HDM-exposed mice using immunofluorescent confocal microscopy, FACS analysis and cell culture. Other projects in the Johnson lab are investigating the molecular mechanisms by which chronic inflammation affects mesenchymal progenitor cell differentiation and migration and assessing the impact of smoke exposure and oxidative stress on pericyte function in COPD
Competitive grants awarded:
UK Medical Research Council New Investigator Research Grant – 2013-2016
Imperial College London Junior Research Fellowship – 2011-2014
Johnson JR*, Folestad E, Rowley JE, Noll EM, Walker SA, Lloyd CM, Rankin SM, Pietras K, Eriksson U and Fuxe J. Pericytes contribute to airway remodeling in a mouse model of chronic allergic asthma.Am J Physiol Lung Cell Mol Physiol. 2015 308(7):L658-71*corresponding author Johnson JR, Nishioka M, Chakir J, Risse P, Almaghlouth I, Bazarbashi AN, Plante S, Martin JG, Eidelman D, Hamid Q. IL-22 contributes to TGF-β1-mediated epithelial-mesenchymal transition in asthmatic bronchial epithelial cells.Respiratory Research 2013 14(1):118 Lavigne F, Petrof BJ, Johnson JR, Lavigne P, Binothman N, Kassissia GO, Al Samri M, Giordano C, Dubé N, Hercz D, Benedetti A, Hamid Q. Effect of topical corticosteroids on allergic airway inflammation and disease severity in obstructive sleep apnoea.Clinical and Experimental Allergy 2013 43(10):1124–1133 Anderberg C, Cunha SI, Zhai Z, Cortez E, Pardali E, Johnson JR, Franco M, Páez-Ribes M, Cordiner R, Fuxe J, Johansson BR, Goumans MJ, Casanovas O, ten Dijke P, Arthur HM, Pietras K. Deficiency for endoglin in tumor vasculature weakens the endothelial barrier to metastatic dissemination. Journal of Experimental Medicine 2013 210(3):563-79 Johnson JR, Roos A, Berg T, Nord M, Fuxe J. Chronic respiratory aeroallergen exposure in mice induces epithelial-mesenchymal transition in the large airways.PLoS One. 2011 6(1):e16175 Vincent T, Neve EP, Johnson JR, Kukalev A, Rojo F, Albanell J, Pietras K, Virtanen I, Philipson L, Leopold PL, Crystal RG, de Herreros AG, Moustakas A, Pettersson RF, Fuxe J. A SNAIL1-SMAD3/4 transcriptional repressor complex promotes TGF-beta mediated epithelial-mesenchymal transition.Nature Cell Biology 2009 11:943-950 Johnson JR, Pacitto SR, Wong JK, Archer EW, Eirefelt S, Miller-Larsson A, Jordana M. Combined budesonide/formoterol therapy in conjunction with allergen avoidance ameliorates house dust mite-induced airway remodeling and dysfunction.American Journal of Physiology: Lung Cellular and Molecular Physiology 2008 295:L780-788 Rydell-Törmänen K*, Johnson JR*, Fattouh R, Jordana M, Erjefält JS. Induction of vascular remodeling in the lung by chronic house dust mite exposure. *equal contributions; featured on the journal coverAmerican Journal of Respiratory Cell and Molecular Biology 2008 39(1):61-67 Johnson JR, FK Swirski, BU Gajewska, RE Wiley, R Fattouh, SR Pacitto, JK Wong, MR Stämpfli and M Jordana. Divergent immune responses to house dust mite lead to distinct structural-functional phenotypes.American Journal of Physiology: Lung Cellular and Molecular Physiology 2007 293:L730-739 Johnson JR, Wiley RE, Fattouh R, Swirski FK, Gajewska BU, Coyle AJ, Gutierrez-Ramos J-C, Ellis R, Inman MD and Jordana M. Continuous exposure to house dust mite elicits chronic airway inflammation and structural remodeling.American Journal of Respiratory and Critical Care Medicine 2004 169:378-385 *featured on the journal cover Full publication list available upon request