.

Dr Anna V Hine


Reader in Molecular Biology


School of Life and Health Sciences
Aston University
Aston Triangle
Birmingham B4 7ET
UK

email: a.v.hine@aston.ac.uk

telephone: +44 (0) 121 204 3961

Research Theme

Cellular and Molecular Biomedicine 

Research Centre

Aston Research Centre for Healthy Ageing (ARCHA) 

Member of the Biology Teaching Programmes
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Career History

Research Interests

  • Protein Engineering
  • Research at the interface between molecular biology and the physical sciences
  • Development of enabling technologies for biomedicines
  • Protein-DNA interactions of Cys2His2 zinc fingers
  • Protein-DNA interactions of single Cys4 zinc fingers

Research Collaborations


Click on links to view the pages of collaborating labs.....


Press Releases

  1. http://www.bbsrc.ac.uk/news/people-skills-training/2013/130402-f-innovators-pt1-hine.aspx
  2. http://www1.aston.ac.uk/about/news/releases/2013/march/aston-academic-wins-commercial-innovator-of-the-year-award/
  3. http://isogenica.com/aston-academic-wins-commercial-innovator-of-the-year-for-work-with-isogenica/
  4. http://isogenica.com/isogenica-secure-funding-from-uks-technology-strategy-board-to-develop-gene-library-synthesis-technology/.

Commercial Products

Isogenica are now providing ProxiMAX randomisation as a commercial service under the trade name ColibraTM.  Please see http://isogenica.com

Membership of Professional Bodies

  • Fellow of the Society of Biology (and Committee Member, West Midlands Branch)
  • Member of the Biochemical Society

Publications

Please note that links to articles below will work only if you have appropriate access. If you are unable to access an article from these links, please email me to request a reprint.    

  • Nagel, D.A, Behrendt, J.M., Chimonoides, G.F., Torr, E.E., Devitt, A., Sutherland, A.J. and Hine, A.V. (2014).

    Polymeric microspheres as protein transduction reagents.
    Mol. Cell Proteomics,13(6), 1543-1551.
    Link to article

  • Ashraf, M., Frigotto, L., Smith, M.E., Patel, S., Hughes, M.D., Poole, A.J., Hebaishi, H.R.M., Ullman, C.G. and Hine, A.V. (2013)
    ProxiMAX randomization: a new technology for non-degenerate saturation mutagenesis of contiguous codons.
    Biochem Soc. Trans. 41(5), 1189-1194.  Link to article

  • Behrendt, J.M., Nagel, D.A, Chundoo, E., Alexander, L.M., Dupin, D., Hine, A.V., Bradley, M. and Sutherland, A.J. (2013)
    Synthesis and Characterization of Dual-Functionalized Core-Shell Fluorescent Microspheres for Bioconjugation and Cellular Delivery.
    PLoS ONE, 8(3): e50713. Link to article 
  • Ashraf,M, Mughes,MD and Hine, AV (2013)
    Oligonucleotide library encoding randomised peptides
    US patent US 8357638 B2 Link to document

  •  Ashraf,M, Mughes,MD and Hine, AV (2012)
    Oligonucleotide library encoding randomised peptides
    European patent EP 2236612 Link to document
  • Ashraf,M, Mughes,MD and Hine, AV (2011)
    Oligonucleotide library encoding randomised peptides
    European patent EP 1907548 Link to document.
  • Barbosa, H.S.C., Hine, AV., Brocchini, S., Slater, N.K.H. and Marcos, J.C. (2010).
    Dual affinity method for plasmid DNA purification in aqueous two-phase systems.
    J. Chromatography A, 1217, 1429-1436.  Link to article
  • Hine, AV., Chen X.F., Hughes M.D., Zhou, K.M., Davies, E., Sugden K., Bennion I., and Zhang L. (2009)
    Optical fibre-based detection of DNA hybridization.
    Biochem. Soc. Trans.
    37, 445-449. Link to article
  • Behrendt, J.M., Afzaal, M., Alexander, L.M., Bradley, M., Hine, A.V., Nagel, D., O'Brien, P., Presland, K. and Sutherland, A.J. (2009)
    Thiol-containing microspheres as polymeric ligands for the immobilisation of quantum dots.
    J. Mater. Chem.19(2), 215-221.  Link to article
  • Barbosa, H., Hine, A.V., Brocchini, S., Slater, N.K.H. and Marcos, J.C. (2008).
    Affinity partitioning of plasmid DNA with a zinc finger protein.
    J Chromatogr. A 1206(2), 105-12.  Link to article
  • Darby, R.A.J., Forde, G.M., Slater, N.K.H. and Hine, A.V. (2007)
    Affinity purification of plasmid DNA directly from crude bacterial cell lysates.
    Biotechnol. Bioeng.
    98(5), 1103-1108. Link to article

  • Chen X.F., Zhang L., Zhou, K.M., Davies, E., Sugden K., Bennion I., Hughes M.D. and Hine, AV. (2007)
    Real-time detection of DNA interactions with long-period fiber-grating-based biosensor.
    Optics Lett.
    32(17), 2541-2543. Link to article

  • McCairn, M.C., Hughes, M.D., Hine, A.V. and Sutherland, A.J. (2006)
    A platform for both solid-phase peptide nucleic acid oligomer synthesis and subsequent in situ detection and quantification of nucleic acid sequences
    J. Comb. Chem. 8
    (5), 639-642 Link to article

  • Forde, G.M., Ghose, S., Slater, N.K.H., Hine, A.V., Darby, R.A.J. and Hitchcock, A.G. (2006)
    LacO-Lacl interaction in affinity adsorption of plasmid DNA
    Biotechnol. Bioeng. 95(1), 67-75. Link to article

  • McCairn, M.C., Hughes, M.D., Hine, A.V. and Sutherland, A.J. (2006)
    Solid-phase PNA synthesis and in situ scintillation proximity assay for the detection of PNA-DNA hybridization
    J. Comb. Chem. 8(1)
    , 1-3. Link to article

  • Darby, R.A.J. and Hine, A.V. (2005)
    LacI-mediated sequence-specific affinity purification of plasmid DNA for therapeutic applications.
    FASEB J. 19(3), 801-803. Link to article


  • Khal, J., Hine, A.V., Fearon, K.C.H., Dejong, C.H.C. and Tisdale, M.J. (2005)
    Increased expression of proteasome subunits in skeletal muscle in cancer patients with weight loss.
    Int. J. Biochem. Cell Biol. 37(10),
    2196-2206. Link to article

  • Hughes, M.D., Zhang, Z.-R., Sutherland, A.J., Santos, A.F. and Hine, A.V. (2005)
    Discovery of active proteins directly from combinatorial randomized protein libraries without display, purification or sequencing: identification of novel zinc finger proteins.
    Nucleic Acids Res.
    33(3), e32, 1-8. Link to article (open access)

  • Khal, J; Wyke, SM; Russell, ST, Hine, AV and Tisdale, MJ (2005)
    Expression of the ubiquitin-proteasome pathway and muscle loss in experimental cancer cachexia
    Brit. J. Cancer
    93(7), 774-780. Link to article

  • Ashraf, M., Hughes, M.D. and Hine, A.V. (2005)
    Oligonucleotide library encoding randomised peptides.
    Patent Publication No. WO 2007010243. Link to esp@cenet

  • Darby, R.A.J. and Hine, A.V. (2004)
    Purification of DNA molecules.
    Patent Publication No. WO2006003355. Link to esp@cenet
  • Zhang, Z.-R., Palfrey, D., Nagel, D.A., Lambert, P.A., Jessop, R.A., Santos, A.F. and Hine, A.V. (2003)
    Fluorescent, microplate-based analysis of protein-DNA interactions I: immobilized protein
    Biotechniques
    , 35, 980-986. Link to article
  • Zhang, Z.-R., Hughes, M.D., Morgan, L.J., Santos, A.F. and Hine, A.V. (2003)
    Fluorescent, microplate-based analysis of protein-DNA interactions II: immobilized DNA
    Biotechniques
    , 35, 988-996. Link to article 

  • Hughes, M.D., Nagel, D.A., Santos, A.F., Sutherland, A.J. and Hine, A.V. (2003)
    Removing the redundancy from randomised gene libraries.
    J. Mol. Biol.
    , 331, 967-972. Link to article

  • McCairn, M., Hine, A.V. and Sutherland, A.J. (2003)
    Synthesis and evaluation of scintillant-containing poly(oxyethylene glycol) polymer (POP-Sc) supports.
    J. Mater. Chem.
    13, 225 - 231. Link to article

  • Khal, J., Hine, A.V. and Tisdale, M.J. (2003)
    Quantitative RT-PCR to investigate the effect of proteolysis inducing factor (PIF) on the expression of ubiquitin-proteasome proteolytic pathway.
    Brit. J. Cancer 88,Supp1, S48.


  • Woodgate, J., Palfrey, D., Nagel, D.A., Hine, A.V. and Slater, N.K.H. (2002).
    Protein-mediated isolation of plasmid DNA by a zinc finger-glutathione S-transferase affinity linker.
    Biotech. Bioeng.
    79(4), 450-456. Link to article

  • Hine A.V., Hughes M.D., Zhang, Z.-R., Nagel, D.A., Ashraf, M. and Santos, A.F. (2002)
    Combinatorial gene libraries and high-throughput screening for rapid protein engineering.
    Abstr. Pap. Am. Chem. Soc.
    224, 325-BIOT Part 1, Aug 18. Request a reprint

  • Hughes, M.D., Nagel, D.A. and Hine, A.V. (2002)
    Efficient gene randomisation for generation of protein libraries.
    Abstr. Pap. Am. Chem. Soc.
    224, 193-BIOT Part 1, Aug 18. Request a reprint

  • Zhang, Z.-R., Hughes M.D, and Hine, A.V. (2002)
    Real-time, high throughput screening of protein-DNA interactions.
    Abstr. Pap. Am. Chem. Soc.
    224, 247-BIOT Part 1, Aug 18 Request a reprint

  • Darby, R.A.J., Slater, N.K.H. and Hine, A.V. (2002)
    Affinity-based isolation of plasmid DNA.
    Abstr. Pap. Am. Chem. Soc.
    224, 251-BIOT Part 1 Aug 18. Request a reprint

  • Hine, A.V., Hughes, M.D., Nagel., D.A., Ashraf, M., Sutherland, A.J. and Santos, A.F. (2002)
    MAX Codon Gene Libraries
    Patent Publication No. US2006269913 Link to esp@cenet

  • Palfrey, D., Picardo, M.. and Hine, A.V. (2000).
    A new randomisation assay reveals unexpected elements of sequence bias in model "randomised" gene libraries: implications for biopanning.
    Gene.
    251(1), 91-99. Link to article

  • Palfrey, D., Cook, P.J., Smythe, J.A., Lip, G.Y.H. and Hine, A.V. (1999).
    Simplified preparation of human arterial sections for PCR analysis of Chlamydia pneumoniae and human DNA.
    J. Clin. Pathol. - Mol. Pathol.
    52, 289-294. Link to article

  • Kusakabe, T., Hine, A.V., Hyberts, S.G. and Richardson, C.C. (1999)
    The Cys4 zinc finger of bacteriophage T7 primase in sequence-specific, single-stranded DNA recognition.
    Proc. Natl. Acad. Sci. USA.
    96, 4295-4300. Link to article

  • Hine, A.V., Morgan, L.J. and Palfrey, D. (1999).
    Zinc fingers, DNA and SPA: the origin of a second genetic code?
    "Set99 - A Showcase of British Science, Engineering and Technology by Young Researchers at the House of Commons" abstr. C25.


  • Hine, A.V., Morgan, L.J., Palfrey, D. and Santos, A. (1998)
    Gene and protein libraries and methods relating thereto
    Patent Publication No.EP1112355. Link to esp@cenet

  • Hine, A.V. and Richardson, C.C. (1994).
    A functional chimeric DNA primase: the Cys4 zinc-binding domain of bacteriophage T3 fused to the helicase of bacteriophage T7.
    Proc. Natl. Acad. Sci. USA
    91, 12327-12331. Link to article

  • Richardson, C.C., Beauchamp, B.B., Debyser, Z., Himawan, J., Hine, A.V., Mendelman, L.V., Mulcahy, H.L., Notarnicola, S.M., Tabor, S. and Yang, X-M (1994).
    Protein interactions at the replication fork of bacteriophage T7.
    Harvard University Committee on Virology 1994 symposium (abstr.)


  • Hine, A.V. and Brown, T.A. (1994).
    Synthesis and expression of a gene encoding a 48-residue repeat in the Pseudomonas syringae ice nucleation protein.
    Gene
    142, 73-78. Link to article

Dr Anna Hine / Aston University / last updated May 2014.

 

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