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Dr Stephane Gross

Lecturer in Cellular Biology

School of Life and Health Sciences
Aston University

Birmingham B4 7ET
UK
Room: MB343A
Tel: +44 (0) 121 204 3467
email: s.r.gross@aston.ac.uk
Contact me by clicking HERE

 

Research Theme

Cellular and Molecular Biomedicine 

Research Centre

Aston Research Centre for Healthy Ageing (ARCHA)  

Dr Stephane Gross
Dr Stephane Gross

Profile

I joined the school of Life and Health Sciences at Aston university as a lecturer in Cell Biology in November 2009 following on from a similar post at Liverpool John Moores University for 2 years (from 2007). It is in the USA that I started being fascinated by the world of Cell Biology when I undertook my postdoctoral studies at the University of Medicine and Dentistry of New Jersey (UMDNJ). It is within these 7 years (2000-2007) that I realised the biological significance of the cellular organisation and their roles in both physiological and diseases states. Prior to this, I received my PhD from the Nottingham Trent University where I graduated in 2000 following on from 3 years of work in the field of Biochemistry. My degrees both from the Nottingham Trent University (applied Biology in 1996) and from Paris XII Creteil (applied biology 1994) were really the steps that took me into the world of science and biology and were the foundations of my career.


Research interests

 The research in our laboratory aims to characterise the role, at the cellular level, of different actin binding/bundling proteins on the organisation of the actin cytoskeleton and the potential roles these proteins may have in disease progressions (such as cancer).

Oncogenic transformation, metastasis, cellular aging and apoptosis are processes where cytoskeleton organization and protein synthesis are often synchronously deregulated. A factor that is essential for regulating both actin remodelling and ribosomal functions is the eukaryotic Elongation Factor 1A (eEF1A). Consequently, and maybe not surprisingly, increases in eEF1A levels have been observed to increase in oncogenic conditions and its level usually correlates to the degree of tumorgenesis. Understanding eEF1A’s non-canonical function towards actin regulation as well as the biological consequences of their interactions on the cytoskeleton and both localised and global protein translation could provide new avenues for therapy in such pathologies. Projects in the lab aim to further determine the importance of the eEF1A-actin interactions on the actin organization in both yeast (saccharomyces cerevisae) and mammalian systems as well as in vitro, using the state of the art facilities in cellular biology and molecular biology offered in our department.

The S100A4 protein has also been linked to cancer progression and has been shown to enhance cellular migration, leading to a metastatic phenotype when it is over expressed in certain tumour cells. Our work and that of others has shown that S100A4 affect the actin cytoskeleton though interaction with the non muscle myosin network. Using cell culture and state of the art facilities in cellular biology and molecular biology, we aim to understand the molecular mechanisms that allow S100A4 to remodel the actin cytoskeleton and the consequences of such reorganisation on cell migration.  

Research funding

2007:    Liverpool John Moores University Promising research Scheme. Awarded £25,000. Role of the elongation factor 1 alpha in actin remodelling and cell migration. 

Qualifications & Education  

  • 1994-1996 Diplome Universitaire de Technologie in Applied Biology, IUT Creteil Paris XII (France) 
  • 1994-1996 BSc. (Hon) Biological Sciences, Nottingham Trent University  
  • 1997-2000 PhD in Biochemistry, Nottingham Trent University  
  • 2007-2008 Postgraduate Certificate in Higher Education, Liverpool John Moores University 

Career History 

  • 2000-2005 Postdoctoral Research Fellow, University of Medicine and Dentistry of New Jersey (USA) 
  • 2005-2007 Adjunct Assistant Professor, University of Medicine and Dentistry of New Jersey (USA) 
  • 2007-2009 Lecturer in Cellular and Molecular Biology, Liverpool John Moores University (UK) 
  • 2009 –     Lecturer in Cellular Biology, Aston University  

PhD Supervision

  • From 2008 : Ms Annette Doyle (Liverpool John Moores University)
  • From 2010 - Present Ms. Connie Goh Then Sin
  • From 2011- Present Ms. Kerry Ann Hallbrook

Selected publications

  • Hipkiss AR, Cartwright SP, Bromley C, Gross SR and Bill RM (2013)
    Carnosine: can understanding its actions on energy metabolism and protein homeostasis inform its therapeutic potential?
    Chem Cent J, in press

  • Gross, S. R. (2013)
    Actin binding proteins: their ups and downs in metastatic life
    Cell adhesion and migration. 7, 2  

  • Du, M. , Wang, G. , Ismail, T. M. , Gross, S.R. , Fernig, D. G. , Barraclough, R. & Rudland (2012)
    S100P dissociates myosin IIA filaments and focal adhesion sites to reduce cell adhesion and enhance cell migration
    Journal of biological chemistry. 287, 19, p. 15330-15344

  • Bawa Z, Bland CE, Bonander N, Bora N, Cartwright SP, Conner MT, Darby RAJ, Dilworth MV, Holmes WJ, Jamshad M, Routledge SJ, Gross SR and Bill RM (2011)
    Understanding the yeast host cell response to recombinant membrane protein production
    Biochem Soc Trans. 2011 Jun;39(3):719-23.

  • Goh, C. , Hersch, N. , Rudland, P. S. , Barraclough, R. , Hoffmann, B. & Gross, S. R. (2011)
    S100A4 downregulates filopodia formation through increased dynamic instability
    Cell adhesion and migration. 5, 5, p. 439-447

  • Wang Z, Collighan RJ, Gross SR, Danen EH, Orend G, Telci D, Griffin M.(2010)
    RGD-independent cell adhesion via a tissue transglutaminase-fibronectin matrix promotes fibronectin fibril deposition and requires syndecan-4/2 and {alpha}5{beta}1 integrin co-signaling.
    J Biol Chem. 17;285(51):40212-29

  • Thamir,  I, Zhang S., Fernig D., Gross S.R. , Martin-Fernandez M.L., Rudland P.S., See V., Tozawa K., Tynan C.J,  Wang G., Wilkinson M.C and Barraclough R (2009).
    Self-association of calcium binding protein, S100A4 and metastasis.
    J. Biol. Chem., In press

  • Gross S.R. and Kinzy T.G. (2007)
     Improper organization of the actin cytoskeleton affects protein synthesis at initiation.
    Mol. Cell. Biology. 27(5):1974-89 

  •   Anand M., Balar B., Ulloque R., Gross S. R. and Kinzy T.G. (2006).
    Domain and nucleotide dependence of the interaction between Saccharomyces cerevisiae translation Elongation Factors 3 and 1A.
    J. Biol. Chem.,281(43):32318-26

  • Chatterjee I., Gross S.R., Kinzy T.G. and Chen K.U.(2006).
    Rapid depletion of mutant eukaryotic initiation factor 5A at restrictive temperature reveals connections to actin cytoskeleton and cell cycle progression.
    Mol. Genet Genomics, 275(3):264-7

  • Gross S.R. and Kinzy T.G. (2005). 
    The translation elongation factor 1A plays essential regulatory functions in the organization of both the actin cytoskeleton and cell morphology.
    Nature Structural and Molecular Biology, 12(9):772-8

  • Komar A.A., Gross S.R., Barth-Baus D., Strachan R., Hensold J.O., Kinzy T.G. and Merrick W.C. (2005).
    Novel characteristics of the biological properties of the yeast Saccharomyces cerevisiae eukaryotic initiation factor 2A.
    J. Biol. Chem., 280(16):15601–11

  •  Anand M., Balar B.A.,  Gross S. R., Ortiz P.A., Ozturk S., Pittman Y.R., Ulloque R.,and Kinzy T.G. (2005).
    The Reactome: Translation Elongation. http://www.reactome.org/cgi-bin/frontpage

  •  Nur-E-Kamal A., Gross S.R., Pan Z., Balklava Z., Ma J. and Liu L.F. (2004)
    Nuclear Translocation of Cytochrome C during Apoptosis.
    J. Biol. Chem.:277(19), 24911-4

  • Gross S.R.*, Mercado M.L.T.*, Nur-E-Kamal A.*, Liu H.Y. *, Movahed R. and Meiners S. (2004).
    Neurite outgrowth by the alternatively spliced region of human tenascin-C is mediated b neuronal a7b1integrin.  
    * Equally contributed to the work
    J. Neurosciences. 24(1):238 –247

  • Gross S.R., Balklava Z. and Griffin M.(2003).
    The importance of tissue transglutaminase in the repair of extracellular matrices and cell death of dermal fibroblasts after exposure to a solarium UVA exposures.
    J. Invest. Dermat.,121(2):412-23
  • Gross S.R.*, Romero A.A.*,Cheng K.Y. Goldsmith N.K. and Geller H.M. (2003).
    An age-related increase in resistance to DNA damage-induced apoptotic cell death is associated with development of DNA repair mechanisms.    * Equally contributed to the work.
    J. Neurochem., 84(6):1275-87.  
  • Balklava Z., Verderio E., Collingham R., Gross S.R., Adams J. and Griffin M. (2002).
    Analysis of tissue transglutaminase function in the migration of Swiss 3T fibroblasts: the active-state conformation of the enzyme does not affect cell motility but is important for its secretion.
    J. Biol. Chem., 277(19):16567-75.

 

  • Verderio E., Gaudry C., Gross S.R., Smith C., Downes S. and Griffin M. (1999).      
    Regulation of cell-surface tissue transglutaminase: effects on matrix storage of       latent transforming growth factor- binding protein-1.
    J
    . Histochem. Cytochem.,    47:1417-1432
  • Verderio E, Nicholas B, Gross S.R., Griffin M.(1998).
    Regulated expression of tissue transglutaminase in Swiss 3T3 fibroblasts: effects on the processing of fibronectin, cell attachment and cell death.
    Exp. Cell Res. 239:119-138

Employable Graduates; Exploitable Research