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Dr Russell Collighan

Lecturer in Molecular Biology
School of Life and Health Sciences,
Aston University
Aston Triangle
Birmingham
B4 7ET

Telephone: +44 (0)121 2044035
Email: r.collighan@aston.ac.uk


Research Group 

Cell and Tissue Biomedical Research

Research Centre

Aston Research Centre for Healthy Ageing (ARCHA) 

Russell Collighan

2009 - Lecturer in Molecular Biology

2004-2009 - Research Fellow, School of Life and Health Sciences, Aston University

1999-2004 - Research Fellow, School of Biomedical and Natural Sciences, Nottingham Trent University

1996-1999 - Higher Scientific Officer, Microbiology department, Veterinary Laboratories Agency (Weybridge)

1992-1995 - PhD Molecular biology, Department of Biochemistry, University of Nottingham

1989-1992 - BSc (Hons) Biochemistry and biological chemistry, Department of Biochemistry, University of Nottingham

Our research is focussed on a group of enzymes called transglutaminases. Transglutaminases are found in many different organisms including microbes, plants and mammals. These enzymes are responsible for a variety of post-translational modifications of proteins, in particular cross-linking of proteins into large molecular weight polymers that are more resistant to degradation. This process is important for the stability of skin, hair, blood clots and the extracellular matrix surrounding cells. Of particular interest is the tissue transglutaminase (TG2), which is important in inflammation, matrix deposition, cell adhesion and cell migration. Consequently, these functions have relevance to a broad range of physiological and pathological processes such as wound healing, tissue fibrosis and scarring, tumour progression, cystic fibrosis, and neurodegeneration.

We have an established programme of research into the molecular mechanisms by which TG2 exerts its physiological and pathological effects. To do this, we use a variety of cell systems which can be engineered to express different site-directed mutant TG2 enzymes that we have produced. We are also developing a range of targeted irreversible inhibitors of TG2 as potential novel therapeutic agents for TG2-associated pathologies. We are also interested in exploiting the matrix cross-linking properties of transglutaminases for the development of stable, biocompatible biomaterials that can be tailored for a variety of biological and physical environments, for example soft tissue or bone repair.

  • Daneshpour, N., Collighan, R., Perrie, Y., Mongeot, A., Griffin, M. (2009). A new approach to the treatment of liver cirrhosis: transglutaminase inhibitors and potential delivery systems. Journal of Pharmacy and Pharmacology 61: A19.

  • Garcia, Y., Naik, H., Collighan, R., Griffin, M., Rodriguez-Cabello, J.C., Pandit, A. (2009). Preferential cellular behaviour is seen with a collagen-elastin-like polypeptide scaffold. Tissue Engineering Part A 14(5):769

  • Daneshpour, N., Collighan, R., Perrie, Y., Telci, D., Mongeot, A., Rathbone, D., Griffin, M. (2008). Investigation of fluorescent derivatives as potential probes for novel transglutaminase inhibitors. Journal of Pharmacy and Pharmacology 60:A56-A57

  • Chau, D.Y.S., Tint, N.L., Joy, S., Collighan R.J., Griffin, M., Rose, F.R.A.J., Dua, H.S. & Shakesheff, K.M. The Development of Bioadhesive Microparticles for Clinical and Regenerative Medical Applications. TERMIS North America 2008 Annual Conference & Exposition.San Diego, US, December 7 - 10, 2008

  • Tint, N.L., Chau, D.Y.S., Collighan, R., Griffin, M., Shakesheff, K.M., Dua, H.S. & Rose, F.R.A.J. Surface Modified Microparticles For Visualisation of Vitreous. European Association for Vision and Eye Research (EVER) 2008 congress, October 1-4 2008, Portoroz, Slovenia

  • Daneshpour, N., Collighan, R., Perrie, Y., Mongeot, A., Rathbone, D., Lambert, P. & Griffin, M. Synthesis of a novel fluorescent transglutaminase inhibitor for controlled release from catheters and other implant devices. 35th Controlled Release Society Annual Meeting and Exposition, New York, 12-16 July 2008.

  • Telci, D., Collighan, R. Basaga, H. & Griffin, M. Nitric oxide is an important regulator of TG2 activity in the extracellular environment by mediating its dual role as matrix crosslinker and cell surface adhesion protein. XXIst Federation of European Connective Tissue Societies (FECTS) Meeting, Marseille, 9-13 July 2008.

  • Chau, D., Collighan, R., Fortunati, D., Garcia, Y., Pandit, A. & Griffin, M. Enzymatically cross-linked collagen as a tissue scaffold. XXIst Federation of European Connective Tissue Societies (FECTS) Meeting, Marseille, 9-13 July 2008.

  • Mongeot, A., Rathbone, D., Collighan, R. & Griffin, M. Novel compounds for reducing matrix accumulation and fibrosis by the inhibition of tissue transglutaminase (TG2). XXIst Federation of European Connective Tissue Societies (FECTS) Meeting, Marseille, 9-13 July 2008.

  • Garcia, Y., Naik, H., Collighan, R., Griffin, M., Rodriguez Cabello, J.C. & Pandit, A. In-vitro Characterization of an Enzymatically Cross-linked Collagen-Elastin Like Polymer Scaffold. 8th World Biomaterials Congress, Amsterdam, The Netherlands, May 28 -  June 1, 2008.

  • Garcia, Y., Collighan, R., Griffin, M. & Pandit, A. Stimulation of Angiogenesis in an Enzymatically Cross-linked Collagen Scaffold. TERMIS EU Meeting, London, Sept. 4-7, 2007.

  • Telci, D., Collighan, R., Li, X., Basaga, H. & Griffin, M. Nitric Oxide may be an Important Regulator of TG2 Activity and Function in the Extracellular Environment. 9th International Conference on Protein Crosslinking and Transglutaminases (PCL9), Marrakech, Morocco, Sept. 1-5, 2007.

  • Garcia, Y., Collighan, R., Griffin, M. & Pandit, A. Stimulation of Angiogensis in a Transglutaminase Cross-linked Collagen Scaffold Used in Dermal Repair. 9th International Conference on Protein Crosslinking and Transglutaminases (PCL9), Marrakech, Morocco, Sept. 1-5, 2007.

  • Mongeot, A., Coutts, I., Saint, R., Rathbone, D., Collighan, R. & Griffin, M. Development of Enzyme-Specific Transglutaminase Inhibitors. 9th International Conference on Protein Crosslinking and Transglutaminases (PCL9), Marrakech, Morocco, Sept. 1-5, 2007.

  • Garcia, Y. Damodaran, G., Collighan, R., Griffin, M. & Pandit, A. Structural and Physicochemical Characterisation of an Enzymatically Cross-linked Collagen Scaffold. Society for Biomaterials 2006 Annual Meeting and Exposition, Pittsburgh, Pennsylvania, USA, April 26-29, 2006.

  • Damodaran, G., Collighan, R., Griffin, M. & Pandit, A. A Collagen scaffold with motif of laminin 5 a 3 chain. Bioengineering In Ireland Conference, Galway, January 27-28, 2006.

  • Halloran, D., Collighan, R., Griffin, M. & Pandit, A. Development Of A Collagen Type II Scaffold For Intervertebral Disc Repair. 8th Annual Meeting of Tissue Engineering Society International. Shanghai, China, 22-25th October, 2005.

  • Parsons, R., Collighan, R., Griffin, M. & Szakacs, G. Biochemical analyses of transglutaminase from Streptomyces. Biocatalysis: Enzymes, Mechanisms and Bioprocesses - a Biochemical Society Focused Meeting in association with Pro-Bio Faraday Annual Conference, 21-22nd November, 2005.

  • Chau, D., Collighan, R., Ye, L., Prince, M., Verderio, E., Addy, V., Ma, X. & Griffin, M. The use of transglutaminase as a biocatalyst in the modification of collagen for the development of biomaterials. 8th International Conference on Protein Crosslinking and Transglutaminases (PCL8), Sept. 1-4, 2005.

  • Parsons, R., Collighan, R., Griffin, M. & Szakacs, G. Biochemical analyses of transglutaminase from strains of Streptomyces. 8th International Conference on Protein Crosslinking and Transglutaminases (PCL8), Sept. 1-4, 2005.

  • Halloran, D., Collighan, R., Griffin, M. & Pandit, A. Development Of A Collagen Type II Scaffold For Intervertebral Disc Repair. 8th International Conference on Protein Crosslinking and Transglutaminases (PCL8), Sept. 1-4, 2005.

  • O’Halloran, D., White, M., Collighan, R.J., Griffin, M. & Pandit, A.S. Scaffold characterisation for nucleus pulposus regeneration. ECM VI/SRNI: Spinal Motion Segment: From Basic Science to Clinical Application, July 4-7, 2005.

  • Garcia, Y., Collighan, R., Griffin, M. & Pandit, A. Cell Viability in an Enzymatically Stabilised Collagen Scaffold. Society for Biomaterials 30th Annual Meeting and Exposition. New applications and Technologies, April 27-30, 2005.

  • Halloran, D., Collighan, R., Griffin, M. & Pandit, A. Development Of A Collagen Type II Scaffold For Intervertebral Disc Repair. Society for Biomaterials 30th Annual Meeting and Exposition. New applications and Technologies, April 27-30, 2005.

  • Halloran, D., Garcia, Y., Broderick, E., Rotchev, Y., Griffin, M., Collighan, R. & Pandit, A. Novel method for stabilising gelatin based scaffolds. Bioengineering In Ireland Conference, January 30-31, 2004.

  • Collighan, R. and Griffin, M. Leather Protein Modification by Transglutaminase.  Pro-Bio Faraday Partnership Annual Conference: “Biocatalysis for Manufacture:  Current Practice and Future Opinions”.  Heriot-Watt University, 5-6 November 2003.

  • Parsons, R. L., Collighan, R. J. & Griffin, M. Isolation, Characterisation and Application of Novel Microbial Protein Cross-Linking Enzymes. Pro-Bio Faraday Partnership Annual Conference: “Biocatalysis for Manufacture:  Current Practice and Future Opinions”.  Heriot-Watt University, 5-6 November 2003.

  • Collighan, R. J. & Woodward, M. J. The SEF14 fimbrial operon of Salmonella enteritidis lies in a pathogenicity island which includes virulence plasmid encoded gene homologues. Society for General Microbiology 142 Ordinary SGM meeting. JAN 5-7, 1999.

  • Collighan, R. J., Bennett, A.J. & Coleman, G. Cloning, mapping and identification of the Aeromonas salmonicida fstA, fepA and irpA genes, encoding the 86, 82 and 74 kDa iron-regulated outer membrane proteins, respectively. 4th International Symposium of the Japanese-Society-of-Fish-Pathology. OCT 03-06, 1997.

  • Collighan, R. J. & Coleman, G. Molecular Biological Studies on the Iron Transport Systems of Aeromonas salmonicidaSeventh International Conference of the European Association of Fish Pathologists: "Diseases of Fish and Shellfish". SEPT 10-15, 1995.

  • Dodsworth, S. J., Collighan, R.J. & Coleman, G. The ability of maltose inducible porin, LamB, antibody to agglutinate Aermonas salmonicida following growth in different carbon sources. Sixth International Conference of the European Association of Fish Pathologists: "Diseases of Fish and Shellfish". SEPT 5-10, 1993.

  • Addy, V., Griffin, M., Collighan, R. & Clara, S. (2005). Enzymatic Treatment of Hides and Leather. WO2005007897

  • Griffin, M., Collighan, R., Chau, D. & Verderio-Edwards, E. (2006). Medical Implant Materials. WO2006027622

  •  Lambert, P.A., Griffin. M., Collighan, R. & Rathbone D.L. (2007). Medical devices and coatings therefor. WO2007010201


Publications